Next-Generation Sequencing (NGS) at Vita Altera Cyprus IVF Center
At Vita Altera Cyprus IVF Center, our commitment to providing outstanding fertility care is rooted in scientific rigor and individualized support. One of the most transformative technologies we offer is Next-Generation Sequencing (NGS)—also referred to as high-throughput DNA sequencing—for comprehensive genetic screening of embryos. By examining each embryo’s genetic structure in depth, we can significantly enhance your likelihood of conceiving a healthy baby.
Understanding NGS in the Context of IVF
During a typical IVF cycle, eggs and sperm are combined under optimal laboratory conditions. The resulting embryos then undergo a process of development, usually lasting 3 to 5 days before transfer. Next-Generation Sequencing fits into this timeline by adding a crucial genetic analysis step, often called PGT-A (Preimplantation Genetic Testing for Aneuploidies).
Key Advantages of NGS
- Comprehensive Chromosomal Analysis: Unlike older methods (e.g., FISH), which focus on limited chromosome sets, NGS looks at all 23 pairs. This breadth offers a full profile of potential numerical or structural abnormalities.
- Rapid & Accurate Results: NGS has evolved to be both faster and more precise, providing actionable insights into each embryo’s genetic makeup.
- Tailored Embryo Selection: By identifying embryos free of chromosomal anomalies, we can prioritize only the healthiest options for transfer, boosting the odds of a successful pregnancy and reducing miscarriage risks.
Relevance to UK Patients
Infertility data from the United Kingdom suggests that 1 in 7 couples faces challenges conceiving naturally. A significant portion of IVF failures stem from undetected chromosomal imbalances within embryos. NGS can serve as a proactive strategy, especially for couples with a history of recurrent implantation failures or miscarriages.
How NGS Integrates with IVF
Ovarian Stimulation & Egg Retrieval
- We begin by stimulating the ovaries through tailored medication protocols. Once follicles mature, we collect the eggs under mild sedation.
- Simultaneously, sperm samples (from a partner or donor) are prepared and assessed for quality.
Fertilization & Early Embryo Culture
- Embryologists fertilize the retrieved eggs, typically via ICSI (Intracytoplasmic Sperm Injection), ensuring optimal sperm-egg interaction.
- Embryos are then cultured in specialized incubators, complete with time-lapse imaging or standard microscopic evaluation.
Embryo Biopsy
- Between Day 3 and Day 5 (often at the blastocyst stage), our embryologists take a small sample of cells—called a “biopsy”—from each viable embryo. This step is critical for Next-Generation Sequencing.
- We place the biopsied cells in a safe, controlled environment for genetic extraction, while the embryo itself remains carefully cultured for potential transfer or freezing.
Genetic Analysis via NGS
- The sampled cells undergo a detailed sequencing process, often performed at specialized genetic laboratories with advanced equipment.
- NGS pinpoints both structural anomalies (like translocations) and numerical abnormalities (e.g., Trisomy 21 linked to Down syndrome).
- Results usually arrive within days, allowing for a timely decision on which embryo(s) to transfer.
Embryo Selection & Transfer
- Once we identify chromosomally normal embryos, we prepare for transfer into the uterus.
- Embryos deemed aneuploid (abnormal) are excluded, thus reducing the risk of miscarriage, failed implantation, or genetic disorders.
- At Vita Altera, we often coordinate a frozen embryo transfer (FET) cycle to align precisely with the patient’s uterine readiness.
Patient Information Confidentiality
Our first priority in all treatment procedures performed at Vita Altera Cyprus IVF Center is patient confidentiality.
Always Contact
Our patients can contact us at any time during the NGS Techniques and ask any questions they may have.
Correct Treatment
Our patients only receive the NGS Techniques they need. This way, they never lose any unnecessary time or money.
What is Analyzed in the Chromosome Test?
NGS examines all chromosomes (23 pairs) for anomalies such as:
- Aneuploidies: Missing or extra chromosomes (e.g., monosomy or trisomy).
- Structural Rearrangements: Inversions, deletions, duplications, and translocations affecting chromosome architecture.
- Potential Mosaicism: The presence of both normal and abnormal cell lines within the embryo.
Comprehensive chromosome screening can identify embryos with the greatest likelihood of leading to a viable pregnancy and healthy birth.
Why Choose NGS?
- Higher Pregnancy & Live Birth Rates: By transferring only chromosomally normal embryos, we directly lower risks of failed implantation and genetic-related miscarriages.
- Fewer Unnecessary Cycles: Couples can avoid repeated IVF attempts with genetically compromised embryos. Targeting normal embryos from the outset is both cost-effective and emotionally supportive.
- Insights into Recurrent Losses: For patients with multiple miscarriages or repeated IVF failures, NGS offers clarity, revealing whether chromosomal factors have played a hidden role.
Procedure Duration and Logistics
- Preparation & Stimulation: 10–14 days of hormonal medication, with frequent ultrasound and blood checks.
- Egg Retrieval: A quick procedure under sedation, typically requiring a single day of clinic attendance.
- Biopsy & Lab Analysis: Completed during embryo culture (Days 3–5). Biopsied cells are shipped or analyzed in-house for NGS.
- Frozen Embryo Transfer (FET): Often a separate cycle, allowing sufficient time to obtain and interpret genetic results.
At Vita Altera Cyprus IVF Center, we collaborate closely with specialized genetics labs, ensuring a swift turnaround. This is particularly helpful for our UK-based patients who need efficient scheduling to balance work, travel, and treatment timelines.
Costs & Considerations for NGS
The investment in Next-Generation Sequencing varies based on:
- Number of Embryos Analyzed
- Complexity of Testing (PGT for aneuploidies only or additional single-gene testing)
- Laboratory Fees
Our team offers transparent discussions of all associated costs, including potential add-ons or repeated cycles, so you can plan accurately. We also help coordinate accommodations and travel should your journey bring you to our clinic from the UK or elsewhere.
Collaborations and Quality Assurance
Vita Altera Cyprus IVF Center frequently partners with recognized genetic testing facilities, including reputable providers with global footprints. Additionally, we maintain advanced embryology labs—often in synergy with the Cyprus Ivf Center—equipped with modern incubators, micromanipulators, and time-lapse imaging systems to support top-tier embryo development.
Data Privacy and Ethical Standards
We adhere to strict confidentiality and ethical guidelines, ensuring each patient’s data remains secure. In line with European best practices, our staff is trained to handle genetic material with sensitivity and meticulous attention to scientific rigor.
Who Should Consider NGS?
- Couples with Recurrent Failures: If you’ve had multiple unsuccessful IVF attempts, NGS can pinpoint chromosomal imbalances that might be causing implantation issues.
- History of Miscarriages: Identifying embryos free from aneuploidies can mitigate the heartbreak of early pregnancy losses.
- Advanced Maternal Age: Women 35 and older experience a higher risk of producing genetically abnormal eggs. NGS helps isolate viable embryos.
- Those Seeking Family Balancing or Additional Genetic Insight: When used alongside other PGT subtypes (e.g., PGT-M for single-gene disorders), NGS offers a comprehensive outlook for embryo viability.
Embark on the NGS Pathway at Vita Altera
While Next-Generation Sequencing is a sophisticated tool, it remains just one element of the fertility journey. Our role at Vita Altera Cyprus IVF Center is to combine advanced technologies with compassionate, patient-focused care, so you feel supported from initial consultation through to embryo transfer and beyond.
If you have questions about how NGS can fit into your unique fertility circumstances—or if you’re a UK resident seeking to optimize your IVF success rate—please get in touch. By merging cutting-edge genetic analysis, individualized treatment planning, and a steadfast commitment to research-driven medicine, we strive to offer you the greatest possible chance of experiencing a healthy and fulfilling pregnancy.
FAQ About NGS
There are many questions about the techniques, we have selected frequently asked questions about this service. If you do not see your answer, please contact us.